Overview

The AssayAgent API provides programmatic access to AI-optimized laboratory assay protocols, quality control parameters, and predictive analytics for assay optimization. This RESTful API is designed to integrate with Laboratory Information Management Systems (LIMS), Electronic Lab Notebooks (ELN), and automation platforms.

Key Features

  • 🧬 500+ Assay Protocols - ELISA, qPCR, Western Blot, Flow Cytometry, NGS, and more
  • 🤖 AI Optimization - Machine learning models trained on millions of assay runs
  • 📊 Predictive QC - Identify potential failures before they occur
  • 🔄 Real-time Updates - Continuously improving protocols based on global data
  • 🔗 Universal Integration - RESTful design works with any modern platform
  • 📈 Analytics Dashboard - Track performance metrics and optimization opportunities

Authentication

The AssayAgent API uses API keys for authentication. Include your API key in the request header:

Request Header
Authorization: Bearer YOUR_API_KEY
Content-Type: application/json

To obtain an API key for the demo environment, use the test key:

Demo API Key
demo_key_2025_assayagent
Security Note: Never expose your API key in client-side code or public repositories. Always use environment variables or secure key management systems.

Base URL

All API requests should be made to:

Production Environment
https://api.assayagent.com/v1
Demo Environment
https://assayagent.com/api

Get Assay Protocol

Retrieve detailed protocol information for a specific assay.

GET /assays/{assay_id}.json 200 OK

Parameters

Parameter Type Required Description
assay_id string Required Assay identifier in format: ASSAY_TARGET (e.g., ELISA_IL6)
optimize boolean Optional Include AI optimization suggestions (default: false)
qc_level string Optional QC stringency: basic | standard | strict (default: standard)

Example Request

cURL
curl -X GET "https://assayagent.com/api/assays/ELISA_IL6.json" \
  -H "Authorization: Bearer demo_key_2025_assayagent"

Example Response

JSON Response
{
  "assay_id": "ELISA_IL6",
  "name": "Human IL-6 ELISA",
  "category": "Immunoassay",
  "version": "2.1.4",
  "ai_optimized": true,
  "confidence_score": 0.94,
  "protocol": {
    "duration_minutes": 240,
    "temperature_celsius": 25,
    "steps": [
      {
        "step": 1,
        "name": "Plate Coating",
        "duration_minutes": 120,
        "temperature": 4,
        "reagents": [
          {
            "name": "Capture Antibody",
            "concentration": "2 µg/mL",
            "volume_ul": 100
          }
        ],
        "ai_tips": [
          "Ensure uniform coating by gentle tapping",
          "Overnight coating at 4°C improves sensitivity by 15%"
        ]
      }
    ]
  },
  "qc_parameters": {
    "cv_threshold": 0.15,
    "r_squared_minimum": 0.98,
    "blank_od_maximum": 0.1,
    "positive_control_range": {
      "min": 1.5,
      "max": 2.5
    }
  },
  "optimization_insights": {
    "success_rate": "94% (based on 12,847 runs)",
    "common_failures": [
      "High background: Check wash buffer preparation",
      "Poor standard curve: Verify dilution accuracy"
    ],
    "recommended_improvements": [
      {
        "modification": "Increase wash cycles to 5x",
        "expected_improvement": "12% reduction in CV"
      }
    ]
  }
}

List Available Assays

Get a list of all available assay protocols in the system.

GET /assays 200 OK

Query Parameters

Parameter Type Description
category string Filter by category (ELISA, qPCR, WesternBlot, etc.)
target string Filter by target molecule
limit integer Number of results (default: 20, max: 100)
offset integer Pagination offset (default: 0)

Error Handling

The API uses standard HTTP status codes and returns detailed error messages:

Status Code Description Common Causes
200 Success Request completed successfully
400 Bad Request Invalid parameters or malformed request
401 Unauthorized Missing or invalid API key
404 Not Found Assay ID does not exist
429 Too Many Requests Rate limit exceeded
500 Internal Server Error Server-side issue (rare)

Error Response Format

{
  "error": {
    "code": "ASSAY_NOT_FOUND",
    "message": "The requested assay protocol was not found",
    "details": "Assay ID 'INVALID_ID' does not exist in the database",
    "timestamp": "2025-08-26T10:30:00Z"
  }
}

Support & Domain Acquisition

This API documentation demonstrates the potential capabilities of an AI-powered laboratory automation platform. AssayAgent.com and all associated intellectual property are available for acquisition.

Ideal for:

  • LIMS/ELN software companies expanding into AI
  • Laboratory instrument manufacturers developing software platforms
  • Biotech startups building next-generation lab automation
  • Clinical diagnostic companies modernizing their infrastructure
Inquire About Domain Acquisition →